Epidemiología de la Proteína de Superficie Neumocócica a (PspA), en Streptococcus pneumoniae Causantes de Infecciones Invasivas en un Hospital Pediátrico de la Argentina
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Se ha puesto énfasis en la Proteína A de superficie neumocócica (PspA) como componente principal para el desarrollo de una vacuna, con el potencial de ofrecer una amplia protección no relacionada a los serotipos. El objetivo de este trabajo fue conocer la frecuencia y distribución de las familias de PspA, 1 y 2 de Streptococcus pneumoniae causantes de infecciones invasivas pediátricas. Se trabajó con 91 aislamientos del período 2005-2012. Se determinó la sensibilidad a los antibióticos conforme al Clinical and Laboratory Standards Institute, el serotipo mediante la técnica de Quellung, y mediante reacción en cadena de la polimerasa las variantes del gen de PspA. De los aislamientos estudiados, 48 (52,7%) fueron identificados como familia 1, 28 (30,8%) como familia 2 y en 6 (6,6%) no se detectó ninguno de los amplicones buscados. La co-expresión de ambas familias se constató en 9 (10%) aislamientos. La familia 2 presentó frecuencias superiores a las esperadas entre los niños < 2 años y en casos de meningitis (p>0,05). Se evidenciaron 16 serotipos diferentes. El serotipo 14 se asoció a ambas familias. No se detectó diferencia entre la distribución de las familias y los resultados de concentración inhibitoria mínima a penicilina y cefotaxima.
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