Optimización de la extracción de antioxidantes y compuestos fenólicos del fruto tarumã

Contenido principal del artículo

Amanda Gentil Polizeli
Luciana Alberti
Luis Antonio Stolz
Larissa Fernandes da Cruz
Liciani Inaê Putti
Luana Bettanin
Georgia Ane Raquel Sehn
Elisandra Rigo
Darlene Cavalheiro

Resumen

El fruto de tarumã (Vitex megapotamica) puede ser una fuente de diversos compuestos bioactivos. En este estudio, su pulpa fue sometida a liofilización con el objetivo de determinar sus compuestos bioactivos, actividad antioxidante y efectos antiinflamatorios, además de optimizar el proceso de extracción acuosa de compuestos fenólicos totales y antioxidantes. Se realizaron análisis de fenólicos totales, flavonoides totales, antocianinas totales, carotenoides, actividad antioxidante y efectos antiinflamatorios. El proceso de extracción fue optimizado según estudios previos. El tarumã resultó ser una fuente potencial de compuestos bioactivos, principalmente compuestos fenólicos, con 5438,59 mg EAG.100 g-1. El diseño experimental mostró que las mejores condiciones incluían un tiempo de extracción de 15 minutos, una relación sólido-líquido de 4,33 g.100 mL-1 y una temperatura de extracción de 90 °C, lo cual es un factor clave para investigaciones futuras. Todos los resultados demuestran el gran potencial del uso de la pulpa de tarumã en futuras preparaciones alimentarias.

Detalles del artículo

Sección

Ingeniería, Tecnología e Informática

Cómo citar

Optimización de la extracción de antioxidantes y compuestos fenólicos del fruto tarumã. (2026). Revista De Ciencia Y Tecnología, 45(1), 16-25. https://doi.org/10.36995/j.recyt.2026.45.002

Referencias

Babotă, M., Frumuzachi, O., Gâvan, A., Iacoviță, C., Pinela, J., Barros, L., Mocan, A. (2022). Optimized ultrasound-assisted extraction of phenolic compounds from Thymus comosus Heuff. ex Griseb. et Schenk (wild thyme) and their bioactive potential. Ultrason. Sonochem. 84, 1–10. https://doi.org/10.1016/j.ultsonch.2022.105954

Bergman, A., Eymael, D. A., Gomes, N. R. Frasson, S. F., Silva, C. S. (2022). Benefits of consuming carotenoids from native Brazilian fruits: a literature review. Rev. Bras. Obes. Nutr. Emagrecimento, 15(97), 1158-1168. (in portuguese). https://www.rbone.com.br/index.php/rbone/article/view/1456

Can-Cauich, C. A., Sauri-Duch, E., Betancur-Ancona, D., Chel-Guerrero, L., González-Aguilar, G. A., Cuevas-Glory, L. F., Moo-Huchin, V. M. (2017). Tropical fruit peel powders as functional ingredients: Evaluation of their bioactive compounds and antioxidant activity. J. Funct. Foods 37, 501–506. https://doi.org/10.1016/j.jff.2017.08.028

Cosmo, N. L., Gogosz, A. M., Nogueira, A. C., Bona, C., Kuniyoshi, Y. S. (2009). Morphology of the fruit, seed and morphoanatomy of the seedling of Vitex megapotamica (Spreng.) Moldenke (Lamiaceae). Acta Bot. Bras. 23(2), 389-397 (in Portuguese). https://doi.org/10.1590/S0102-33062009000200010

Fragoso, M. F., Romualdo, G. R., Vanderveer, L. A., Franco-Barraza, J., Cukierman, E., Clapper, M. L., Carvalho, R. F., Barbisan, L. F. (2018). Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells. Food Chem. Toxicol 121, 237–245. https://doi.org/10.1016/j.fct.2018.08.078

Ghazzawi, H. A., Al-Sayyed, H. F., Al-Kurd, R. A., Mwalla, M. M., Arafat, T. A., AbdelQader, S. M. (2021). Effect of different extraction solvents on the antioxidant content and capacity of nine seasonal fruits. Clin. Nutr. Open Sci. 38, 33–42. https://doi.org/10.1016/j.nutos.2021.06.003

Gómez-Maqueo, A., García-Cayuela, T., Welti-Chanes, J., Cano, M. P. (2019). Enhancement of anti-inflammatory and antioxidant activities of prickly pear fruits by high hydrostatic pressure: A chemical and microstructural approach. Innov. Food Sci. Emerg. Technol. 54, 132–142. https://doi.org/10.1016/j.ifset.2019.04.002

Gomori, G. (1995). Preparation of Buffers for Use in Enzyme Studies. Method Enzymol. 1, 138-146. https://doi.org/10.1016/0076-6879(55)01020-3

Gualberto, N. C., de Oliveira, C. S., Nogueira, J. P., de Jesus, M. S., Araujo, H. C. S., Rajan, M., Leite neta, M. T. S., Narain N. (2021). Bioactive compounds and antioxidant activities in the agro-industrial residues of acerola (Malpighia emarginata L.), guava (Psidium guajava L.), genipap (Genipa americana L.) and umbu (Spondias tuberosa L.) fruits assisted by ultrasonic or shaker extracti. Food Res. Int. 147, 110538. https://doi.org/10.1016/j.foodres.2021.110538

Hazra, B., Biswas, S., Mandal, N. (2008). Antioxidant and free radical scavenging activity of Spondias pinnata. BMC - Complementary and Alternative Medicine, 8(63), 1–10. https://doi.org/10.1186/1472-6882-8-63

Ju, Z.Y., Howard, L.R. (2005). Subcritical water and sulfured water extraction of anthocyanins and other phenolics from dried red grape skin. J. Food Sci. 70(4), 270-276. https://doi.org/10.1111/j.1365-2621.2005.tb07202.x

Kita, A., Rytel, E., Miedzianka, J., Turski, W. A., Wicha-Komsta, K., Kucharska, A. Z., Lenartowicz, T. (2023). The content of biologically active compounds in potato tubers of Ismena (yellow flesh) and Provita (purple flesh) varieties – A comparison. J. Food Compos. Anal. 115, 104898. https://doi.org/10.1016/j.jfca.2022.104898

Liu, Y., Schouten, R. E., Tikunov, Y., Tikunov, Y., Liu, X., Visser, R. G. F., Tan, F., Bovi, A., Marcelis, L. F. (2022). Blue light increases anthocyanin content and delays fruit ripening in purple pepper fruit. Postharvest Biol. Technol. 192, 112024. https://doi.org/10.1016/j.postharvbio.2022.112024

Monteiro, L. K., Vicentini Schaefer, S., Fischer, C., Orellana Luvizotti, A., Rigo, E., Sehn, G. A. R., Cavalheiro, D. (2024). Bioactive compounds of pulp powder of tarumã fruits (Vitex megapotamica) at two maturity stages. Cad. Cie. Agrar. 2024:16. https://doi.org/10.35699/2447-6218.2024.46538

Monteiro, L. K., Fischer, C., Bettanin, L., Rigo, E., Sehn, G. A. R., Zanella, O., Cavalheiro, D. (2024). Extraction of bioactive compounds from Vitex megapotamica: A study and selection of variables. J. Foods Process Eng. 47(5):14623. https://doi.org/10.1111/jfpe.14623

More, P. R., Jambra, A. R., Arya, S. S. (2022). Green, environment-friendly and sustainable techniques for extraction of food bioactive compounds and waste valorization. Trends Food Sci. Tech. 128, 296–315. https://doi.org/10.1016/j.tifs.2022.08.016

Onofre, S. B., Abatti, D., Tessaro, A. A., Tessaro, A. B. (2016). Total Phenolic and Flavonoid Content and Antioxidant Activity of Vitex Montevidensis (Spreng) Moldenke - Verbenaceae. Ciênc. Nat. 38(3), 1197. https://doi.org/10.5902/2179460X21363

Oroian, M., Escriche, I. (2015). Antioxidants: Characterization, natural sources, extraction and analysis. Food Res. Int. 74, 10–36. https://doi.org/10.1016/J.FOODRES.2015.04.018

Pan, L. H., Chen, L. P., Wu, C. L., Wang, J. F., Luo, S. Z., Luo, J. P., Zheng, Z. (2022). Microencapsulation of blueberry anthocyanins by spray drying with soy protein isolates/high methyl pectin combination: Physicochemical properties, release behavior in vitro and storage stability. Food Chem. 395, 133626.https://doi.org/10.1016/j.foodchem.2022.133626

Resende, L. M., Franca, A. S., Oliveira, L.S. (2019) Buriti (Mauritia flexuosa L. f.) fruit by-products flours: Evaluation as source of dietary fibers and natural antioxidants. Food Chem. 270, 53–60. https://doi.org/10.1016/j.foodchem.2018.07.079

Rufino, M. S. M., Alves, R. E., Brito, E. S., Morais, S. M., Sampaio, C. G., Pérex-Jiménez, J., Calixto, F. D. S. (2007). Scientific methodology: Determination of total antioxidant activity in fruits by capturing the ABTS+ free radical. Technical communication 128. Embrapa Agroindústria Tropical, Fortaleza. Access in: https://www.embrapa.br/busca-de-publicacoes/-/publicacao/426954/metodologia-cientifica-determinacao-da-atividade-antioxidante-total-em-frutas-pela-captura-do-radical-livre-abts.

Saini, A., Panesar, P. S., Bera, M. B. (2019). Valorization of fruits and vegetables waste through green extraction of bioactive compounds and their nanoemulsions-based delivery system. Bioresour Bioprocess. 6(26). https://doi.org/10.1186/s40643-019-0261-9

Santos, G. M. dos, Maia, G. A., Sousa, P. H. M. de, Costa, J. M. C. da, Figueiredo, R. W. de, Prado, G. M. do, (2008). Correlation between antioxidant activity and bioactive compounds in commercial açaí pulps (Euterpe oleracea Mart). Arch. Latinoam. Nutr. 58(2), 187–192. (in potuguese). Available in: https://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0004-06222008000200011

Savi, P. D. R. S., Dos Santos, L., Gonçalves, A. M., Biesek S, De Lima CP (2017) Analysis of total flavonoids present in some conventional and organic fruits and vegetables most consumed in the southern region of Brazil. Demetra. 12(1), 275–288. (in portuguese). https://doi.org/10.12957/demetra.2017.22391

Sharma, K., Ko, E. Y., Assefa, A.D., Ha, S., Nile, S.H., Lee, E.T., Park, S.W. (2015). Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties. J. Food Drug Anal. 23(2), 243–252. https://doi.org/10.1016/j.jfda.2014.10.005

Sims, D.A., Gamon, J.A. (2002). Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages. Remote Sens. Environ. 81(2–3), 337–354. https://doi.org/10.1016/S0034-4257(02)00010-X

Singleton, V., Orthofer, R., Lamuela-Raventós, R. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods Enzymol. 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1

Tabaszewska, M., Najgebauer-Lejko, D., Zbylut-Górska, M., Skoczylas., Tokarczyk, G. (2022). Effect of hawthorn berry pre-treatment and preservation methods on the extractability of color-determining compounds and selected antioxidative substances. LWT. 173, 114297. https://doi.org/10.1016/j.lwt.2022.114297.

Vasco, C., Ruales, J., Kamal-Eldin, A. (2008). Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem. 111(4), 816–823. https://doi.org/10.1016/j.foodchem.2008.04.054

Wang, L., Clardy, A., Hui, D., Gao, A., Wu, Y. (2019). Antioxidant and antidiabetic properties of Chinese and Indian bitter melos (Momordica charantia L.). Food Biosci. 29, 73–80. https://doi.org/10.1016/j.fbio.2019.03.010.

Zhao, S., Park, C.H., Yang, J., Yeo, H. J., Kim, T. J., Kim, J.K., Park, S.U. (2019). Molecular characterization of anthocyanin and betulinic acid biosynthesis in red and white mulberry fruits using high-throughput sequencing. Food Chem. 279, 364–372. https://doi.org/10.1016/j.foodchem.2018.11.101

Zhishen, J., Mengcheng, T., Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64(4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2